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KMID : 0613820190290111173
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Journal of Life Science
2019 Volume.29 No. 11 p.1173 ~ p.1178
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Characterization of Agarase from a Marine Bacterium Agarivorans sp. BK-1
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Ahn Byeong-Ki
Min Kyung-Cheol
Lee Dong-Geun
Kim Andre
Lee Sang-Hyeon
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Abstract
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The purpose of this study was to isolate an agar-degrading marine bacterium and characterize its agarase. Bacterium BK-1, from Gwanganri Beach at Busan, Korea, was isolated on Marine 2216 agar medium and identified as Agarivorans sp. BK-1 by 16S rRNA gene sequencing. The extracellular agarase, characterized after dialysis of culture broth, showed maximum activity at pH 6.0 and 50℃ in 20 mM Tris-HCl buffer. Relative activities at 20, 30, 40, 50, 60, and 70℃ were 67, 93, 97, 100, 58, and 52%, respectively. Relative activities at pH 5, 6, 7, and 8 were 59, 100, 95, and 91%, respectively. More than 90% of the activity remained after a 2 hr exposure to 20, 30, or 40℃; about 60% of the activity remained after a 2 hr exposure to 50℃. Almost all activity was lost after exposure to 60 or 70℃ for 30 min. Zymography revealed three agarases with molecular weights of 110, 90, and 55 kDa. Agarose was degraded to neoagarobiose (46.8%), neoagarotetraose (39.7%), and neoagarohexaose (13.5%), confirming the agarase of Agarivorans sp. BK-1 as a β-agarase. The neoagarooligosaccharides generated by this agarase could be used for moisturizing, bacterial growth inhibition, skin whitening, food treatments, cosmetics, and delaying starch degradation.
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KEYWORD
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Agar-degrading bacterium, Agarivorans sp. BK-1, β-agarase, marine bacterium, neoagarooligosaccharides, zymogram
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